Taggd

Taggd


The authors have declared that no competing interests exist. This can be problematic since there can be errors introduced in the barcodes during both their synthesis and sequencing, resulting in non-unique barcodes. Generating unique barcodes is challenging in a number of ways. Introduction Parallel processing of samples is a powerful method to perform multiple experiments in time and cost-effective manner. Operations can be substitutions, insertions or deletions.

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Taggd. What is taggd?.

Taggd


The authors have declared that no competing interests exist. This can be problematic since there can be errors introduced in the barcodes during both their synthesis and sequencing, resulting in non-unique barcodes. Generating unique barcodes is challenging in a number of ways. Introduction Parallel processing of samples is a powerful method to perform multiple experiments in time and cost-effective manner. Operations can be substitutions, insertions or deletions. Taggd

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3 thoughts on “Taggd”

  1. Each barcode has to be unique. As the two operations of filtering and insertion are independent, a worker thread is created to handle the generation of barcodes and push those to a common pool as shown in Figure 1.

  2. Firstly, barcodes should conform to particular constraints such as length, GC-content, cross-hybridisation with experimental sequences, absence of particular restriction enzymes to minimise their interference with the actual experiment. Given the current developments in sequencing platforms, the scale of multiplexing will grow requiring larger numbers of barcodes due to high sequence throughput [3].

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